Microfluidics and BioMEMS Laboratory

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Introduction to Microfluidics

Microfluidics is to study fluids in microscale. The field involves (1) microfabrication (to make devices containing microscale features), (2) microflows (to study fluids, e.g., pumping, valving, mixing, and simulation), and (3) applications.

Devices are fabricated using techniques similar to those used for fabricating computer chips. The techniques include film deposition, patterning (as in photolithography), etching, assembly, and others.  The process of making a thermoplastic device is illustrated in Figure 1.

 

Figure 1. The process to fabricate a plastic microfluidic device. (J. MEMS, 15, 2006, 1060)

 

Manipulation of fluids in a microfluidic device needs pumps, valves, and/or other components.  One of mechanisms suitable for pumping fluids in microdevices is electroosmosis (EO).  EO is generated in a microchannel filled with an appropriate solution when a voltage is applied across the channel. Syringe pumps are often used as well. Microvalves are another key component in a microfluidic device and they may regulate flows, contain fluids, and isolate one region from the other. 

Applications of microfluidics and BioMEMS devices include cell culture and analysis, clinical diagnostics, environmental monitoring, DNA and protein analysis, and biological warfare agent detection. Examples of extensive reviews on the topic include: Special issue in Nature, Vol 442, July 27, 2006, 367-418; Manz, et al. Analytical Chemistry 2010, 82, 4830–4847

Selected Research Projects in Dr. Fan's Lab

Circulating Tumor Cells (CTCs). The goal of this research is to develop microfluidic devices for isolating tumor cells circulating in peripheral blood. CTCs have been explored as biomarkers for cancer diagnosis and prognosis. However, CTCs are extremely rare, typically a few among billions of healthy blood cells.  Therefore, cell sorting and enrichment are required. The background information can be found in the book entitled "Circulating Tumor Cells: Isolation and Analysis". The project has been funded by National Cancer Institute (NCI), McJunkin Family Charitable Foundation, and the University of Florida.

Microfluidic Valves. The goal is to develop thermally actuated and manufactuable microfluidic valves for a variety of applications. The valves can be integrated in a device; the actuation of valves will be controlled by a printed circuit board (PCB). The valves are expected to be useful in any system that needs flow controls. The project has been funded by Defense Advanced Research Projects Agency (DARPA), and a project for an immunoassay array involving a number of microvalves has been funded by National Institute of Health (NIH).

Protein Synthesis Array. The goal is to develop a multiplexed, cell-free protein expression system for high-throughput protein production, so that the functional studies of proteins can be matched with gene-discovery in terms of the scale and throughput. The system consists of an array of microfluidic units and each unit functions as a microreactor to synthesize proteins corresponding to a gene of interest. The array can also be used for detecting biological warfare agents that inhibit protein synthesis such as ricin. The project has been funded by DARPA and Covitect Inc.

Paper Microfluidics. The goal is to develop low-cost, paper-based, rapid analytical devices for point-of-care applications. Laminated paper-based analytical devices (LPADs) are fabricated in a way similar to making an identification card; lamination with plastic sheets increases the mechanical strength and overall durability of a paper device. A project aiming for developing such a device for pathogen detection after an air sampler is currently funded by National Science Foundation (NSF). A project aiming for developing a point-of-care device for Zika virus detection is currently funded by Florida Department of Health (FL DoH).

Secondhand Smoke (SHS). The goal is to develop a hand-held analyzer for detecting SHS exposure. Child exposure to SHS is associated with sudden infant death syndrome, acute respiratory infections, ear problems, and childhood asthma. As a result, a hand-held analyzer will help diagnose a child with an asthma attack or severe ear infection whether SHS is causing the problem. The analyzer consists of components to enrich and detect biomarkers associated with tobacco smoke. The project has been funded by Flight Attendant Medical Research Institute (FAMRI) and James and Esther King Biomedical Research Program of FL DoH.

2D Gel Chip. The goal is to design and fabricate a microfluidic device that provides an alternative approach to two-dimensional gel electrophoresis, which is extensively used in chemical and biological labs. The microfluidics-enabled method likely leads to rapid analysis, high separation resolution, improved protein identification accuracy, and enhanced reproducibility. The device could be used as a tool for biomarker discovery and screening. The project has been funded by Army Research Office and NIH.

Hydrogen Sensor. The goal is to develop a sensor for detecting possible leakage of hydrogen—the fuel of today's space vehicle and possibly tomorrow's automobiles. The sensor is based on an enzyme-catalyzed reaction, which can be carried out in a microfluidic device. Compared to the state-of-the-art, metal or alloy-based hydrogen gas sensors, the benefits include ambient temperature operation and enhanced selectivity over background gases. The project has been funded by NSF and National Aeronautics and Space Administration (NASA).

Examples of Corporations Using Microfluidics

Agilent Technologies; Caliper Life Sciences (now PerkinElmer); Cepheid; Fluidigm; Gyros; Micralyne; Micronics; Microfluidic ChipShop and the others listed in this website.

Useful Links:

Several scientific journals are dedicated to the field, including Lab on a chip by Royal Society of Chemistry; Microfluidics and Nanofluidics by Springer; Biomicrofluidics by American Institute of Physics (AIP); and Journal of MEMS, by the Institute of Electrical and Electronics Engineers (IEEE) and American Society of Mechanical Engineers (ASME). Other scientific journals related to the filed include Analytical Chemistry, Sensors and Actuators A, Sensors and Actuators B, Biosensors and Bioelectronics, Journal of Micromechanics and Microengineering, etc.

Check Chips & Tips for solutions to common practical problems encountered in the lab.

Many scientific conferences dedicated to the field, including µTAS, Transducers, Hilton Head Workshop, MEMS, Society for Laboratory Automation & Screening (SLAS) Meeting, Microtechnologies in Medicine and Biology, etc.